Effect of low-magnitude high frequency vibration on osteogenic differentiation of human periodontal ligament stem cells

doi:10.19439/j.sjos.2019.05.006

Abstract

Abstract: PURPOSE: To investigate the effect of low-magnitude high frequency vibration (LMHFV) on proliferation, migration ability and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs). METHODS: hPDLSCs were isolated from premolar and randomized into vibration culture group (magnitude：0.3 g; frequency：40 Hz; time：15 min/24 h) and static culture group. CCK-8 was used to identity the proliferation of hPDLSCs. Wound-healing assay was used to evaluate migration ability of hPDLSCs. The osteogenesis gene expression was analyzed by RT-PCR, and the osteogenesis protein expression was analyzed by Western blot. The osteogenesis differentiation capability was evaluated by alizarin red staining. The data were analyzed using SPSS 21.0 software package. RESULTS: After LMHFV, the proliferation and migration ability of hPDLSCs were increased. The expression level of RUNX2, ALP, Col-1, and OCN was significantly augmented under LMHFV. Alizarin red staining and Western blot proved the same trend. CONCLUSIONS: The results demonstrate that LMHFV can promote hPDLSCs proliferation, migration ability and osteogenic differentiation.

Key words: Vibration, Periodontal ligament stem cells, Proliferation, Osteogenic differentiation

CLC Number:

R781.4

BAI Kun-hong, HU Cheng, LI Qing-qing, WANG Fei. Effect of low-magnitude high frequency vibration on osteogenic differentiation of human periodontal ligament stem cells[J]. Shanghai Journal of Stomatology, 2019, 28(5): 478-483.

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